Poll-The et al., "The enigmatic role of tafazzin in cardiolipin metabolism," Biochimica etBiophysica Acta, vol.
Koehler, "The cardiolipin transacylase, tafazzin, associates with two distinct respiratory components providing insight into Barth syndrome," Molecular Biology of the Cell, vol.
Our previous study showed that tafazzin knockdown enhances mitochondrial reactive oxygen species (ROS) production in neonatal cardiac fibroblasts .
Our current study was designed to demonstrate that mitochondrial ROS play critical roles in tafazzin knockdown induced cardiac and mitochondrial dysfunction in cultured cardiac myocytes.
The antibody against tafazzin was from Santa Cruz (Santa Cruz, CA).
Total RNA isolation from NVMs and real-time RT-PCR with the primers for tafazzin and [beta]-actin in an Eppendorf RealPlex2 were performed as we described previously .
ACMs were plated on laminin coated coverslips, incubated with tafazzin shRNA adenovirus for 24 h and treated with mito-Tempo overnight.
Tafazzin Knockdown Enhanced Mitochondrial ROS Production, which Is Abrogated by the Mitochondrial Antioxidant.
Mitochondrial Antioxidant Normalized ATP Decline Induced by Tafazzin Knockdown.
Mitochondrial Antioxidant Prevented Protein Kinases Activation Induced by Tafazzin Knockdown.