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* TBARS: TBARS is a measurement of lipid peroxidation.
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The PM steaks demonstrated greater (p<0.05) TBARS than LL counterparts on days 5 and 9 (Table 1), whereas the lipid oxidation was not detected in both muscles on day 0.
The mean values of the oxidative stress marker in the form of the total lipid peroxides level (TBARS [|M/l]), as determined in the plasma of the experimental and control subjects, amounted to 2.25 [+ or -] 0.60 and 1.19 [+ or -] 0.26, respectively.
The resultant pink colour was the index of TBARS. Plasma TBARS was stated as nmol/ml.
To establish a lipid peroxidation index, Yagi's (14) method was effective to identify the levels of thiobarbituric acid-reactive substances (TBARS).
Shortly thereafter, to evaluate the degree of oxidative stress by the TBARS method using protocol adapted from Lapenna and collaborators (2001), blood was centrifuged at 800 X g for 15 minutes.
Malondialdehyde is a component of thiobarbiturate acid-reactive substances (TBARS) and the final product of lipid peroxidation.
The fasting period had no significant effect on the hepatic thiobarbituric acid reactive substances (TBARS), but, it caused a significant decrease in the hepatic catalase and glutathione peroxidase (p<0.05).
This reaction is called ''lipid peroxidation'' and can be measured as thiobarbituric acid-reactive substances (TBARS) to show the degree of lipid peroxidation in an organism.
Measurement of thiobarbituric acid reactive substances (TBARS)
The extent of oxidation was determined using the thiobarbituric acid reactive substances (TBARS) assay.
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