Response of the antioxidant defense system to tert-butyl hydroperoxide
and hydrogen peroxide in a human hepatoma cell line (HepG2).
Annexin-FITC: Fluorescein isothiocyanate coupled to annexin V DCFDA: 2',7'-Dichlorodihydrofluorescein diacetate DHE: Hydroethidine PI: Propidium iodide MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide ROS: Reactive oxygen species t-BHP: tert-Butyl hydroperoxide
. Competing Interests
Caption: Figure 6: Results of ultra-weak photon emission measurements after mixing 0.5 ml of 10 mM tert-Butyl hydroperoxide
with 1 ml of deionized water.
Gonzo, "Catalytic reactivity of Cr/Si[O.sub.2] in the liquid phase oxidation of cyclohexanol by tert-butyl hydroperoxide
," Reaction Kinetics and Catalysis Letters, vol.
Augusto, "In vivo metabolism of tert-Butyl hydroperoxide
to methyl radicals.
(TBHP, oxidant) and sodium formaldehyde sulphoxylate (SFS, reductant) were received from Roma Organics, India and Demosha Chemicals Pvt.
5A), but less than the known strong oxidant tert-butyl hydroperoxide
These may suggest that a suitable feed of styrene is 1.5 mol/kg rubber for the graft-copolymerization of styrene onto U-DPNR with tert-butyl hydroperoxide
of 3.3 x [10.sup.-5] mol/g rubber and tetraethylenepentamine at 30[degrees]C.
dissolved in olive oil was used as a standard compound for POV analysis.
It has been proposed that tBHP reacts with Fe + leading to the formation of tert-butyl hydroperoxide
(tB[O.sup.*]) radicals leading to lipid peroxidation [23, 26, 27].
Thus, we decided to test whether low concentrations of EGCG would alter mitochondrial dysfunction caused by tert-butyl hydroperoxide
For chemiluminescence (CL) measurement, reaction mixtures were placed in 20 mL scintillation vials (low-potassium glass) containing final concentrations of plasma (250 [micro]L), 30 mM K[H.sub.2]P[O.sub.4]/[K.sub.2]HP[O.sub.4] buffer (pH 7.4), and 120 mM KCl with 3 mM of tert-butyl hydroperoxide
in a final volume of 2 mL.