Plasma and serum proteins were digested with trypsin in a buffer system containing Trifluoroethanol
Estrada, "Xylanase II from Trichoderma reesei QM 9414: conformational and catalytic stability to chaotropes, trifluoroethanol
, and pH changes," Journal of Industrial Microbiology and Biotechnology, vol.
To the polymer solution was added sodium trifluoroethoxide prepared by the treatment of trifluoroethanol (0.86 g, 8.63 mmol) with NaH (0.35 g, 8.63 mmol, 60% in mineral oil) in THF (50 mL).
To a THF solution of poly(dichlorophosphazene) (1.0 g, 8.63 mmol) was added two reagent solutions simultaneously: a THF solution (50 mL) of sodium 1H,1H,5H-perfluoropentan-1-oxide, prepared by the reaction of 1H,1H,5H-perfluoropentan-l-ol (2.60 g, 11.22 mmol) and NaH (0.45 g, 11.22 mmol, 60% in mineral oil), and a THF solution (50 mL) of sodium trifluoroethoxide prepared by the treatment of trifluoroethanol (1.12 g, 11.22 mmol) with NaH (0.45 g, 11.22 mmol, 60% in mineral oil).
 Nonstandard abbreviations: A1AT, [alpha]-1-antitrypsin; SERPIN, serine protease inhibitor; COPD, chronic obstructive pulmonary disease; IEF, isoelectric focusing; MS, mass spectrometry; LC-MS/MS, liquid chromatography-tandem MS; TFE, trifluoroethanol
; IAA, iodoacetamide; TFA, trifluoroacetic acid; DTT, dithiothreitol; FCS, fetal calf serum; Z 3-16, Zwittergent detergent 3-16; DMF, dimethylformamide; LOQ, limit of quantitation; MRM, multiple reaction monitoring.
EVALUATION OF TRIFLUOROETHANOL AND UREA AS DENATURANTS
To completely denature the proteins in plasma, we compared a protocol that used trifluoroethanol (26) with one that used a more traditional approach using urea.
 Nonstandard abbreviations: LC-MS/MS, liquid chromatography-tandem mass spectrometry; MRM, multiple reaction monitoring; apoB, apolipoprotein B; apoA-I, apolipoprotein A-I; TFE, trifluoroethanol; SISCAPA, stable isotope standards and capture by anti-peptide antibodies.
ABBREVIATIONS CBC 1,1'-Carbonyl-Bis-Caprolactam CPL Caprolactam DSC Differential Scanning Calorimetry PA Polyamide PCPA Polycarbonate/Polyamide blend ROP Ring Opening Polymerization TFE 2,2,2 Trifluoroethanol
Briefly, 10 [micro]L of human serum was dried and then resuspended in 25 [micro]L of 25 mmol/L ammonium bicarbonate, 25 [micro]L trifluoroethanol
, and 2.5 [micro]L of 200 mmol/L DTT.
Polymer chemically unattached to the fibers (KEVP150) was extracted by immersing fibers in trifluoroethanol for 36 h and then filtering.
It can be recalled that only five passes were done in the dichloride and the diamine, and that washing with trifluoroethanol removed all chemically unbonded molecules.