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References in periodicals archive ?
The pellet was re-suspended in 35l of Tris-EDTA (TE) buffer.
En el laboratorio de bioquimica genetica del instituto de investigaciones geneticas esta tecnica es realizada con dos ml de sangre con EDTA; la cual es tratada con solucion salina y tetracloruro de carbono, para realizar una corrida electroforetica en una lamina de acetato humedecida previamente con buffer tris-EDTA al 10%, que luego se colorea con para evidenciar la presencia de los tipos de hemoglobina.
Mainstay of the project was cellulose acetate electrophoresis using Tris-EDTA - Borate buffer at a pH of 8.
Briefly, plugs were prepared by incubating washed cells in Tris-EDTA buffer solution (pH 8.
8) The test is based on use of tris-EDTA to permeabilize a bacterial cell and release of [beta] lactamases into the external environment.
Epitope retrieval was achieved by heating the deparaffinized tissue slides for 20 minutes in Envision Flex Target Retrieval Tris-EDTA pH 9.
Finally, replacement of the aqueous environment with a good nucleic acid solvent such as low salt, buffered substance (such as Tris-EDTA, pH 8 "TE8") allows the now purified nucleic acids to release from the silica and come back into solution for recovery.
Briefly, an aliquot of 50 [micro]L of plasma was mixed with 1 mL of Tris-EDTA at 1 mM, and the first reading was at 412 nm (A1 reading).
La extraccion se realizo por el metodo fenol cloroformo alcohol isoamilico y precipitacion con etanol y acetato de sodio, el precipitado se resuspendio en 30 [micron]l de Tris-EDTA pH: 8,0 (Tris HCl 10 mM, EDTA 0,1 M) y se almaceno a 4[grados]C (modificado de GOODWIN & LEE, 1993.
The pelleted DNA was centrifuged at 15000rpm for 15 min then dissolved with 100 [micro]l of Tris-EDTA buffer (10 mM Tri- Buffer pH 7.