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References in periodicals archive ?
The test is based on use of Tris-EDTA to permeabilize a bacterial cell and release [beta]-lactamases into the external environment.
The pellet was re-suspended in 35l of Tris-EDTA (TE) buffer.
The pellet was then air dried followed by addition of Tris-EDTA. Storage was done at -20degC.
En el laboratorio de bioquimica genetica del instituto de investigaciones geneticas esta tecnica es realizada con dos ml de sangre con EDTA; la cual es tratada con solucion salina y tetracloruro de carbono, para realizar una corrida electroforetica en una lamina de acetato humedecida previamente con buffer tris-EDTA al 10%, que luego se colorea con para evidenciar la presencia de los tipos de hemoglobina.
The precipitated DNA was washed in 70% ethanol and dried at room temperature and then resuspended with 40 [micro]L of TE (Tris-EDTA; 10 mM Tris-HCl, lmM EDTA) + RNAse (10[micro]g/mL).The DNA quality was visually estimated in 0.8% agarose gels, stained with ethidium bromide and observed under UV light.
Mainstay of the project was cellulose acetate electrophoresis using Tris-EDTA - Borate buffer at a pH of 8.9.
Briefly, plugs were prepared by incubating washed cells in Tris-EDTA buffer solution (pH 8.0) with 10 mL lysozyme (10 mg/mL) before mixing with 1.5% molecular grade agarose and 0.17 mg/mL proteinase K (Roche, Mannheim, Germany) (5).
The bacterial pellet was washed in 1.5 mL of 0.85% NaCl, centrifuged for 2 minutes at 12,000 g, and was resuspended in 0.4 mL Tris-EDTA buffer (T10E25).
Tris-EDTA buffer pH 7.5 (TE buffer), 1M Tris- HCl, 50 mM Tris-HCl pH 7.5 (elution buffers) were prepared as (Sambrook and Russell, 2001).
Epitope retrieval was achieved by heating the deparaffinized tissue slides for 20 minutes in Envision Flex Target Retrieval Tris-EDTA pH 9.0 (Dako, Carpinteria, California) using a steamer.