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References in periodicals archive ?
All the tubes are not formed precipitate then do subcultures on media Trypticase Soy Agar media (TSA) and incubated in a C[O.sub.2] incubator at 37[degrees]C for 24 hours.
The number of cells adhered to each coupon (CFU [cm.sup.-2]) was determined by transferring serial dilutions to Petri dishes (surface plating) that contained trypticase soy agar (TSA) and incubating the Petri dishes at 35[degrees]C[+ or -]1[degrees]C for 48h (ESPER, 2010).
A single, well-isolated colony from each trypticase soy agar plate was then suspended in 5 mL of trypticase soy broth and incubated at 37[degrees]C until the inoculum achieved 0.5 McFarland standard ([greater than or equal to] 0.10D at 620 nm).
coli in PBS after HHP treatment was determined by the pour culture method in Trypticase Soy agar (TSA, Becton Dickenson and Company).
Logphase cultures of WT growing aerobically in trypticase soy broth (TSB) were treated with NG.
Aliquots of 50-[micro]L remnant blood culture specimens were enriched in 1 mL of trypticase soy broth with 0.5% sodium chloride, Lim Broth (Todd-Hewitt broth with colistin nalidixic-acid, Becton Dickinson), or chopped meat broth (Becton Dickinson), depending on metabolic requirements.
A loopful of test organisms was inoculated in 10 mL of trypticase soy broth with 1% glucose in test tubes.
Table 1-Specified Microbial Limits Total Microbial Count Raw Materials Finished Products 10 cfu/g or ml 2 - 100 cfu/g or ml 6 6 300 cfu/g or ml - 3 1000 cfu/g or ml 6 1 10000 cfu/g or ml - - Other (not specified) 1 - Table 2 - Protocols (Methods) for Microbial Detection Raw Materials Finished Products Dipstick 1 1 USP * 3 2 Plate Count ** 7 9 Enrichment *** 2 3 Rapid Method *** - 3 * United States Pharmacopeia 61 Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests ** Trypticase soy agar, Cetrimide, Sabouraud, MacConkey and Potato dextrose agars *** Letheen, Tetrathionate, Lauryl tryptose, Modified tween letheen and "MLAT" broths.
Axenic and monoxenic cultivation of Acanthoamoeba in serum, thioglycollate broth, trypticase soy broth and brain heart infusion broth was also performed (Penland and Kirk, 1997).
They formulated laboratory media (trypticase soy broth) with the different preservatives at pH 7.
coli (~[10.sup.7] CFU), After 1, 14, and 24 h incubation, the bunches were washed in 30 ml of saline, sonicated (UP400S, 24 kHz, Hielscher GmbH: 50% amplitude, 100% pulse) for 20 s (to detach the bacteria from the fibers), and plated to a dilution of up to [10.sup.-4] in duplicate on trypticase soy agar contact plates (VWR).
According to Gardam and Miller (1998), the combination of Trypticase soy agar and sheep blood agar is recommended for the presumptive identification of Streptococcus pneumoniae.