UCBUUmbilical Cord Blood Unit
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All content (25 mL) of each UCBU thawed was obtained by puncture with a hypodermic syringe.
A total of 5193 UCBU were collected in the NCBT cord blood bank.
The electrophoretic profiles of the DNA products obtained after PCR amplification using specific primers for ERIC sequences were determined for all strains obtained from contaminated UCBU. The electrophoretic analysis of PCR reaction products (amplicons) of the evaluated strains revealed that the number of bands ranged from 2 to 12 in different profiles.
Thus, the clinical benefit could be achieved only when two UCBUs were transplanted: one unit expanded in culture to shorten time to neutrophil engraftment and the second un-manipulated unit to provide the long and durable engraftment.