For example, endopinacocytes stained intensely with WGA, moderately with Con A, but did not stain with SBA, PNA, or UEA-1
. Choanocytes displayed a particular affinity for Con A and less so for other lectins, but mesohyl cell stained intensely with most of the lectins.
Lectin histochemistry (8) was carried out with the following horseradish peroxidase-conjugated (HRP) lectins: soybean agglutinin (SBA) with specificity for N-acetyl-d-galactosamine (GalNac), wheat germ agglutinin (WGA) for N-acetyl-glucosamine (GlcNAc), peanut agglutinin (PNA) for [beta]-galactose (Gal), Ulex europaeus agglutinin (UEA-1) for [alpha]-l-fucose (Fuc), concanavalin A (Con A) for [alpha]-mannose (man), and Limulus polyphemus agglutinin (LPA) for sialic acid (SA).
1a,b), as well as WGA, Con A, PNA, and UEA-1. However, acid treatment did not enhance LPA staining, suggesting that sialic acid is not cryptic, but is terminal and, therefore, separate from sugar chains with sulfated termini.