UEILUnion Indépendante de l'Industrie Européenne des Lubrifiants
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Essentially, pulp sections were labelled with a mixture of a polyclonal antibody to human TRPV1 raised in rabbit (1:2000; GlaxoSmithKline, Harlow, UK) and either: i) a monoclonal antibody to the general neuronal marker, human protein gene product 9.5 (PGP 9.5) raised in mouse (1:1000; Ultraclone, Isle of Wight, UK) or ii) a mixture of biotinylated Ulex europaeus agglutinin 1 lectin (UEIL) (1:100; Vector Laboratories, Peterborough, UK), and an antibody raised in mouse against human alpha smooth muscle actin (.SMA) (1:50; Novocastra Ltd, Newcastle Upon Tyne, UK).
Vascular expression of TRPV1 was determined by calculating the percentage area of staining for this label within tissue also labelled for UEIL +/- SMA.
In toward ower weoued ne beode ye na mon for te bihalden, ah yef his deuotiun bit hit ant haued grant, drahed ow wel inward ant te ueil adun toward ower breoste, ant sone dod pe clad ayein ant fesmid hete-ueste, yef he loked toward bed oder easked hwer ye ligged, ondsweried lihtliche, "Sire, per-of wel mei duhen," ant halded ow stille.