UPEPUrine Protein Electro-Phoresis
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SPEP and UPEP were performed on the SPIFE 3000 electrophoresis analyzer (Helena Laboratories).
Both UPM and UPEP are nonstationary series, as is the interest rate.
For a patient's results to be analyzed for variation, at least 1 method had to fulfill the definition of a measurable response criterion (3): (1) SPEP M-spike [greater than or equal to] 10 g/L, (2) UPEP M-spike [greater than or equal to] 200 mg/24 h, or (3) iFLC [greater than or equal to] 100 mg/L in the presence of an rFLC that was not with in reference intervals.
The results of UPEP and urine immunoelectrophoresis studies were negative; urine total protein excretion levels were within normal limits.
Urine protein electrophoresis (UPEP) [4] showed the presence of an unusual spike in the [beta] region (Fig.
Twenty-four-hour urine collection was significant for a total protein of 0.54 g/24 h (reference interval, <0.15 g/24 h); urine protein electrophoresis (UPEP) and immunofixation revealed 2 monoclonal A light chain bands.
We read with interest the recent paper on the combination of serum protein electrophoresis (SPEP) and serum free light chain (FLC) assay as a potential alternative to SPEP and urine protein electrophoresis (UPEP) screening for paraproteinemia (1), as well as the accompanying editorial on sensitivity and specificity issues of the serum FLC assay (2).
Retrospective studies have shown that serum FLC assays are more sensitive than serum protein electrophoresis (SPEP) or urine protein electrophoresis (UPEP) for the detection of these 3 diseases.