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Plasma pentraxin 3 (PTX3) levels were measured in each 100 [micro]l sample by a standard ELISA apparatus at 450 nm by using a PTX3 kit (Uscn Life Science Inc., Wuhan, China).
Serum levels of leptin and resistin were assessed using ELISA kits (USCN Life Science and Technology, Wuhan, China).
Serum prostatein concentrations were determined by using ELISA kit assay (SEE174Hu; USCN, Wuhan, China) according to the manufacturer's instructions.
Plasma adiponectin and leptin concentrations were determined using a validated sandwich enzyme-linked immunosorbent assay (Uscn Life Science Inc., Wuhan, China) with an adiponectin- or leptin-specific antibody.
Ltd., China; leptin: Uscn Life Science Inc., China; Insulin/TSH/T3/T4: Demeditec Diagnostics GmbH, Kiel, Germany) in Laboratory of Department of Biochemical, Faculty of Veterinary Medicine, Istanbul University.
These authors used commercial ELISA assay kits from Cusabio Biotech and USCN Life Sciences, both assays ostensibly directed toward mature human Ucn2 (19, 20).
Serum concentration of aldosterone and interleucine-6 was determined by the Enzyme Linked ImmunonoSorbent Assay (ELISA) method using commercial kits (UScn, Life Science Inc.).
Enzyme-linked Immunabsorbent Assay Kit for Myelin Basic Protein (Uscn Life Science Inc.
Serum Total Antioxidant Status (TAS) Concentration has been determined by kits which was purchased from Randox laboratories Ltd., Serum, Alpha Fetoprotein (AFP) has been determined by kits which was purchased from Uscn life Sience Inc.
The analyses were carried out by using enzyme-linked immunosorbent assay kit by Cloud-Clone Corp US, assembled by Uscn Life Science Incorporated, China.
Nesfatin-1 concentration was determined using a commercially available ELISA kit (USCN Life Science Inc.