UVRAUtah Valley Radiology Associates (Provo, UT)
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We performed multilocus sequence analysis (MLSA) on housekeeping genes (clpA, clpX, nifS, pepX, pyrG, rplB, recG, and uvrA) as described (29; online Technical Appendix Table 1, https://wwwnc.cdc.gov/EID/article/24/9/171381-Techapp1.pdf).
We obtained similar results using 7 housekeeping loci (Figure 3; online Technical Appendix Tables 4, 5) and, in particular locus uvrA. For this locus, sequences of Candidatus B.
The Maxicell technique was then applied to a variety of protein identification projects and allowed Sancar to rapidly identify the proteins encoded by the uvrA, uvrB and uvrC genes.
In 1983, Sancar used the purified uvrA, uvrB and uvrC proteins to reconstitute essential steps in the NER pathway.
coli AB1157 (wild-type) and AB1886 (uvrA deficient) in the exponential growth phase ([10.sup.8] cells/mL, 2-3 h, 37[degrees]C) were grown from stocks in the stationary growth phase.
A total of 50 samples were amplified across all eight housekeeping genes (clpA, clpX, nifS, pepX, pyrG, recG, rplB, and uvrA) [7] and were submitted for sequencing.
Amplification of the eight housekeeping genes (clpA, clpX, nifS, pepX, pyrG, recG, rplB, and uvrA) was performed with primers and nested touchdown PCR conditions for clpA, nifS, pepX, pyrG, recG, and uvrA as previously described [7, 8].
The uVRA includes pre-configured set-up for protection of Windows and Linux servers and can autoprovision virtual machines at recovery time for instant fail-over.
coli (WP2 uvrA) both with and without metabolic activation.
coli WP2 uvrA -S9 1.00 [+ or -] 1.00 0.00 [+ or -] 0.00 +S9 1.00 [+ or -] 1.00 0.67 [+ or -] 0.58 EGMAX Strain S9 19.5 [micro]g/ml 39.0[micro]g/ml S.
Salmonella typhimurium TA100, TA1535, TA98, and TA1537 and Escherichia coli WP2 uvrA were used as test strains.