GUS Histochemical Assay: The expression of uidA
gene was studied through histochemical X-Gluc assay.
Fourteen strains were assigned as "unknown" because they were positive for the four genes arpA/chuA/ yjaA/TspE4.C2, and were screened for the uidA
gene, confirming the detection of E.
coli strains were isolated from 120 different samples (Table 2) and confirmed by amplifying 146 bp product of uidA
gene with single step polymerase chain reaction (Figure 1).
Liberibacter-free transgenic plants were produced by MIYATA (2009) using Agrobacterium tumefaciens with three binary vectors containing the uidA
gene under control of three different phloem-specific promoters: AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A.
Y pues nos otros, a los quales son propuestos tan marauillosos gualardones dela nuestra uida
, que nin la grandeza dellos se puede dezir nin la muchedunbre contar, ?auemos de creer que, dormiendo seguros y occiosos, assi en la uida
actiua como en la contenplatiua, auemos de alcancar ninguna dellas conla mano?
E.coli were identified by 23-bp forward primer and 21pb reverse primer, targeting the uidA
gene of to obtain 162 bp product (Fig.
For example, the use of the [beta]-glucuronidase (GUS) encoding reporter gene (uidA
) allows histochemical localisation of gene expression .
In methanogens, both lacZ and uidA
(encoding [beta]-glucuronidase from E.
(2005) reportaron la transformacion genetica de los cultivares 'Kirkagac 637' y 'Noi Yarok', mediante Agrobacterium utilizando la cepa EHA105 conteniendo el plasmido pME504 con los genes de seleccion nptII, bar y uidA
, probando diversos medios y concentraciones de kanamicina.