BZA, either as monotherapy or in association with estrogens, induces the smallest effect on uterine wet weight
in immature rats and ovariectomized mice or rats in comparison with controls treated with vehicle or with raloxifene and lasofoxifene [8, 22, 32].
Effects on the Uterine Wet Weight
and Uterine Content Parameters.
However, uterine wet weight
did not significantly increase with D3 treatment at 24 hr.
The luminal epithelial height and uterine wet weight
are well-established end points for evaluating estrogenicity of chemicals (Branham et al.
Neonatal mice treated orally on days 2-5 with 25 mg/kg GIN showed increased uterine wet weight
gain similar to mice that received 20 mg/kg GEN sc, thus indicating that approximately 80% of orally administered GIN reached sufficient circulating levels of the active compound to elicit a biological effect compared with sc GEN (Jefferson et al.
As expected, treatment with increasing doses of [E.sub.2] resulted in increased uterine wet weight
. In both strains, perinatal treatment with 250 ng BPA/kg bw/day did not alter this parameter (Table 2).
Effect of diet on uterine/ovarian and uterine wet weight
and uterine luminal epithelial cell height.
(2001) found a significant increase in ER transcriptional activity in the adult uterus after exposure to 1 mg/kg BPA, whereas this dose did not induce a uterine wet weight
Finally, the in vivo estrogenic bioactivity of the used polycarbonate cages was tested by measuring the uterine wet weight
of prepubertal female mice housed in the cages.
MXC, at 100 mg/kg/day, increased uterine wet weight
and epithelial height to about one-third that of [E.sub.2] 2 [micro]g/kg/day.
We determined uterine wet weight
or DNA content as previously described (14).
These doses of DES and estradiol have been previously determined to cause maximum uterine wet weight