V136valine polymorphism at codon 136
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1C, the T137 variant can easily be distinguished from both wild-type (A136) and the risk-associated variant V136.
The F141 variant could be set to the penultimate position of the probe, with a minor destabilizing effect compared with V136 but with sufficient difference to be seen the LightCycler.
To make your reservation, you can either book by calling the Stena Line reservations number on 01 204 7777 quoting V136.