As the most potential live biotherapeutic agent, vaccinia virus (VACV
) have been used for in vitro and in vivo studies, and clinical trials.
Human infections with OPVs frequently emerge or reemerge, including cowpox virus in Europe (4), monkey-pox virus in Africa and North America (5), buffalopox in India (6), and VACV
in South America (7), especially in Brazil (8).
Vaccine had strains of VACV
, that cross reacts with other members of the Poxviridae family, including the smallpox virus (DAMON, 2007; FENNER et al., 1988).
(1987) who stated that BPXV intra-cytoplasmic inclusion bodies of B-type were similar to inclusion bodies of CPXV, however, these showed dissimilar appearance from Guarnieri bodies found in case of VACV
, that were plentiful, small, asymmetrical, granulated and eosinophilic.
He reported a needle stick in his left thumb had occurred on November 17 while he was inoculating a mouse by scarification with VACV
. He had no fever, chills, or other systemic or neurologic symptoms.
Primary ovine fetal turbinate cells (OFTu) were used to amplify ORFV IA-82, Madin-Darby bovine kidney (MDBK) cells were used for the amplification of BoHV-1, and Vero cells were used to amplify VACV
. Cells were grown in Eagle's minimum essential medium (MEM) supplemented with 10% fetal bovine serum (FBS, Nutricell, Brazil), 100 U/mL penicillin, and 100 [micro]g/mL streptomycin, and maintained at 37[degrees]C and 5% C[O.sub.2].
Polar compounds are expected to be responsible for the antiviral activity of the EE extract which has disclosed significant effect against HHV-1, VACV
and DEN 2.
After molecular analyses of the ha gene, a close relation of Cantagalo virus and VACV
used in vaccination campaigns against smallpox in Brazil has been demonstrated.
Over the past 17 years in Brazil, many zoonotic outbreaks of vaccinia virus (VACV
) infection have been recorded throughout the country, becoming a burden for the dairy industry and public health (2).
The molecular analysis, quantitative PCR (qPCR) for VACV
, was performed on samples macerated in 20% (w:v) suspension in Minimal Eagle Medium (MEM), and 2% antibiotics (Potassium Penicillin G 11,200IU/mL; Streptomycin 0.01g/ mL, Gentamicin 0.01g/mL, L-Glutamine 0.029g/ mL and Amphotericin B 0.5mg/mL).
Importantly, this approach has also been utilized to study cell surface molecules targeted by vaccinia virus (VACV
Vaccinia virus (VACV
) is the live viral component of smallpox vaccine.