We observed a significant interaction of well-water As with ICAM1 rs128432 and VCAM1 rs3176867 for CVD on both multiplicative and additive scales.
We observed significant interactions of well-water As with rs281432 in ICAM1 and rs3176867 in VCAM1 for CVD.
Among the GIH population (Gujarati Indians in Houston, Texas) in the Hapmap 3 SNP data set, ICAM1 rs28l432 was not found, and no proxy SNPs were identified for VCAM1 rs3176867 (SNAP; Johnson et al.
We estimated significant interactions of As exposure with ICAM1 rs281432 and VCAM1 rs3176867 on CVD.
ICAM1 and VCAM1 polymorphisms, coronary artery calcium, and circulating levels of soluble ICAM-1: the Multi-Ethnic Study of Atherosclerosis (MESA).
RT-PCR was performed for ICAM1, VCAM1, CCL2, BCL2, BAX and CASP9.
Effects of EGCG and L-theanine on TNF-[alpha]-induced ICAM1, CCL2 and VCAM1 gene expression in cultured endothelial cells
Since several molecules such as endothelial ICAM1, CCL2 and VCAM1 mediate monocyte adhesion to endothelial cells, we next examined the effects of EGCG and L-theanine on ICAMI, CCL2 and VCAM1 expression using RT-PCR.
Effects of EGCG on TNF-[alpha]-induced increases in VCAM1 protein levels in cultured endothelial cells
We used Western blotting to examine the protein expression levels of VCAM1 in endothelial cells following TNF-[alpha] treatment.
Furthermore, this study also showed that EGCG blocked the IL-1[beta]-induced expression of VCAM1.
Abbreviations EC epicatechin ECG epicatechin gallate ECC epigallocatechin EGCG epigallocatechin-3-gallate eNOS endothelial nitric oxide synthase HO-1 heme oxygenase-1 ICAM1 intercellular adhesion molecule-1 JNK c-Jun N-terminal kinase HUVEC human umbilical vein endothelial cells 3-MA 3-Methyladenine MCP1 monocyte chemoattractant protein-1 NO nitric oxide PAI-1 plasminogen activator inhibitor-1 ROS reactive oxygen species TNF-[alpha] tumor necrosis factor alpha VCAM1 vascular cell adhesion molecule-1 VEGF vascular endothelial growth factor vWF von Willebrand factor