IF-IF genotype was positively associated with the risk of AMI in subject above 45 years after adjusting for potential confounders [OR = 9.
A few recent studies have demonstrated an association of serum levels of 25(OH)D and risk of T2DM,8,9 suggesting that the VDBP
polymorphism could be playing some role in the pathogenesis of T2DM.
Thus, the objective of this analysis was to quantify the short-term (6-week) within-person variability of fasting serum 25(OH)D, FGF23, VDBP
, free 25(OH)D, bioavailable 25(OH)D, PTH, calcium and phosphorus in a community-based population.
Few published studies, evaluating the role of VDBP
exon 11 polymorphisms in the susceptibility to T1D, gave inconsistent results (15-17).
With its family-friendly themes and visual comedy, the universal appeal of the VDBP
footage has no language barrier and has been used in marketing campaigns across the globe including France, Norway, Taiwan, and Korea.
When developed and applied properly, such methods are not likely to be affected by sample-to-sample differences in VDBP
assay used in this work (R&D Systems) is intended only for research purposes and has not been validated to the standard required for diagnostic use.
In addition to this information, it is also imperative to realize that while some studies have proved that vitamin D2 is 30% to 50% less effective that vitamin D3 due to its less binding potential with VDBP
, (48), (49) others (50) show that vitamin D2 is as effective as vitamin D3 in maintaining and raising serum 25(OH) D concentrations.
The automated 25OHD methods, which have necessarily abandoned solvent extraction or protein precipitation to free 25OHD from binding proteins, are likely to be more susceptible to variations in VDBP
However, competition between the 25OH-D capture antibody and VDBP
in patient samples makes these assays difficult to control.
We obtained polyclonal rabbit antihuman antibodies against VDBP
, albumin, TTR, CysC, B2M, and SAP from Dako.
These results are not surprising because the selectivity of VDBP
is highly dependent on the extraction/purification techniques used before sample incubation with the reactive protein.