VVS2

AcronymDefinition
VVS2Very Very Slightly Imperfect 2 (diamonds)
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Eighty-nine alleles were obtained, ranging from 11 (VVS2) to 14 (VVMD31 and VVMD7), with an average and effective number of 12.7 alleles per locus (Table 1).
The most frequent alleles in each locus were VVMD5 - 238 (25.8%), VVMD7 - 239 (21.6%), VVMD27 - 185 (26.7%), VVMD31 - 212 (29.2%), VVS2 - 133 (20.0%), VrZAG62 - 189 (35.0%) and VrZAG79 - 255 (21.7%) (Figure 2).
Alleles Locus Number He (1) (%) PIC (2) (%) [F.sub.maximum] (3) VVMD5 12 83.9 81,9 25.8 VVMD7 14 87.4 86,1 21.6 VVMD27 13 83.6 81,7 26.7 VVMD31 14 83.3 81,4 29.2 VVS2 11 85.9 84,3 20.0 VrZAG62 12 81.8 80,1 35.0 VrZAG79 13 88,1 87,1 21.7 Media 12,7 84,8 83,2 (1) Expected heterozygosity; (2) polymorphic information content; (3) Maximum frequency of alleles.
Flawless or internally flawless (FL, IF) diamonds are priced roughly 13% higher than VVS1 and VVS2 diamonds, more than 30% higher than VS1 or VS2 diamonds, and almost 60% higher than SI1 or SI2 diamonds.
They come in and ask for a VVS2, Rappaport Report gem with G to H color.'" (quoting a jeweler)); W.
No sentido de facilitar a comparacao com os dados da literatura e com os bancos de dados internacionais, as amostras foram genotipadas com os dez loci microssatelites mais difundidos pela comunidade cientifica internacional: VVS2 (THOMAS e SCOTT, 1993), VVMD27 (BOWERS et al., 1996), VVMD5, VVMD7, VVMD25, VVMD28, VVMD31, VVMD32 (BOWERS et al., 1999b), VrZAG62 e VrZAG79 (SEFC et al., 1999) (Tabela 1).
O programa utilizado para reacao de PCR (Polymerase Chain Reaction) foi: (i) desnaturacao do DNA e ativacao da Taq DNA polimerase a 95[degrees]C, por 7 minutos (GoldTaq) ou 3 minutos (BioTaq); (ii) 35 ciclos de amplificacao distribuidos em 45 segundos a 94[degrees]C, 45 segundos a 50[degrees]C (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62 and VrZAG79) ou 56[degrees]C (VVMD25, VVMD28, VVMD31 and VVMD32), 1 minuto e 30 segundos a 72[degrees]C; (iii) extensao final de 7 minutos a 72[degrees]C, e (iv) resfriamento a 4[degrees]C.
Temperatura de Tamanho Anelamento do alelo Loci Referencia ([degrees]C) (bp) VVS2 Thomas & Scott (1993) 50 129-155 VVMD5 Bowers et al.
The strong diamond section is carried by an important 17.71 carat cut-cornered rectangular D colour, VVS2 clarity, diamond ring, signed by J.