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References in periodicals archive ?
Lupus anticoagulant confirmation testing was considered positive when the difference between both aPTT-LA clotting times was greater than 8 seconds ([aPTT-LA.sub.Difference], Staclot LA [Diagnostica Stago S.A.S, Asnieres sur Seine, France]) or the ratio between the neat diluted Russell viper venom time (dRVVT) and the [dRVVT.sub.Confirm] ([dRVVT.sub.Ratio], Staclot DRVV Screen/Staclot DRVV Confirm) was greater than 1.25.
b, [dRVVT.sub.Confirm] (diluted Russell viper venom time confirmation testing).
LA cannot be measured directly, and a series of tests are used to support or rule out the presence of autoantibodies, including prothrombin time (PT), activated partial thromboplastin time (APTT), and dilute Russell's viper venom time (DRVVT).
And we are one of the few manufacturers of the dilute Russell's viper venom time test (dRVVT) in the world.
LAC was detected again on admission with the following results: kaolin clotting time greater than 200 seconds (normal range 100 to 160), dilute Russell Viper Venom Time 96 seconds (normal range 0 to 45), Russell Viper Venom+Phospholipid Time 50 seconds (normal range 0 to 38) with a resultant dilute Russell Viper Venom/Russell Viper Venom+Phospholipid Time index of 1.3 (normal range 0 to 1.3).
Inhibitor screening results for the presence of a lupus anticoagulant Assay Baseline results 72 hour results (seconds) (seconds) PTT-LS 58.9 100.3 PTT-LS 1:1 mix -- 58.5 DVVT 55.3 66.8 DVVT (1:1) -- 47.0 DVVT ratio -- 1.02 (ratio only) STACLOT-LA 1 -- 62.7 STACLOT-LA 2 -- 52.4 STACLOT-LA difference -- 10.5 PTT-LS = APTT lupus sensitive reagent DVVT = dilute Russell's viper venom time; DWT ratio of greater than 1.3 is indicative of an LA 1:1= mixing studies with rabbit normal pooled plasma STACLOT LA = confirmatory high phospholipid assay STACLOT LA difference is amount of correction.
Laboratory data showed hemoglobin 33 g/L, leukocyte 23.6 x [10.sup.9]/L, a prolonged activated partial thromboplastin time [is greater than] 150 seconds (control [is less than]42 seconds) and prothrombin time of 26.1 seconds (control 14.0 seconds), International Normalized Ratios value = 3D 2.09, and the presence of lupus anticoagulant (LA) antibodies (prolonged Russell viper venom time and confirmed by the STACLOT LA ELISA test, Reaads Medical Products, Inc.).
However, her laboratory tests deserve some comments: there was a strong presence of LA according to the dilute Russell's viper venom time (DRWT), but the activated partial thromboplastin time (APTT) was within the reference interval.
(12,13) In previous studies, prolongation of the dPT was found to be associated with higher risk of thrombosis, (14) and receiver operating characteristic curve analysis indicated that the dPT ratio might be more useful for predicting thrombosis than diluted Russell viper venom time. (10) Generally, a prothrombin-based assay performed with diluted thromboplastin has high sensitivity and specificity for the presence of LA, caused by both anti-[beta]2GPI and anti-prothrombin antibodies.
ABBREVIATIONS: APL = antiphospholipid antibodies; APS = antiphospholipid syndrome; APTT = activated partial thromboplastin time; [[beta].sub.2]-GPI = [beta.sub.2]-glycoprotein I; dRVVT = dilute Russell's viper venom time; ELISA = enzyme-linked immunosorbent assay; Ig = immunoglobulin; KCT = kaolin clotting time; LA = lupus anticoagulant.
The use of the dilute Russell viper venom time for the diagnosis of lupus anticoagulants.
Six of 46 samples identified as having a LA were SCT-negative; in these patients, the only test positive was the diluted Russell venom time. Six of 91 LA-negative patients were positive by SCT Confirm assay.