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To find these "potent responders," tests for mean response greater than the detection limit of the assay are performed using weighted t-tests with the same weighting scheme used for WNLS [see Supplemental Material, p.
Compounds with significant fits to the Hill model using either NIS or WNLS, but not both, are classified as INCONCLUSIVE* [[+ or -]3].
* Fit the four-parameter Hill model better than a horizontal line (no response) in either the unweighted (NLS) or weighted (WNLS) curve fit.
Regression models are ordinary least squares (OLS), weighted ordinary least squares (WOLS), nonlinear least squares (NLS) and weighted nonlinear least squares (WNLS).
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