WRPISWestern Regional Plant Introduction Station (US)
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According to ARS entomologist Steve Clement, public and private research organizations in the United States and abroad are increasingly mining the WRPIS grass collection to identify more nontoxic strains.
Very few cereal leaf beetles survived after eating leaves from endophyte-infected wild tall fescue (Lolium arundinaceum) accessions originating from North Africa and stored at WRPIS.
The objectives of this study are (i) to compare [N.sub.e]/[N.sub.c] among different harvest methods, (ii) to test inflorescence sampling among numerous grass species at the WRPIS, and (iii) to predict the optimum number of inflorescences to sample to most efficiently increase [N.sub.e].
Estimates of [N.sub.e]/[N.sub.c] were made based on seed number per plant from three accessions, one each from three perennial grass species maintained at the WRPIS. The three entries were F.
([double dagger]) WRPIS = (National Plant Germplasm System) Western Region Plant Introduction Station, Pullman, WA; FRRL = Forage and Range Research Laboratory, Logan, UT; numbers indicate year of original (o) seed acquisition or year of last seed increase (i).
Johnson returned to the WRPIS as a USDA-ARS Research Agronomist responsible for the management of extensive germplasm collections of forage and turf grasses, perennial legumes, and safflower.
Estimates of [N.sub.e]/[N.sub.c] were made on the basis of seed number per plant from three accessions of three model perennial grass species maintained at the WRPIS. The three species were Festuca pratensis, Lolium perenne, and Pseudoroegneria spicata, and each species was represented by three accessions.
The plots were incorporated into the grass regeneration nurseries at the WRPIS, and each plot was isolated by at least 25 m from other accessions of the same species.
Original and regenerated seed of this collection is stored at -88 [degrees] C at the WRPIS. Storage at this temperature will likely retain endophyte viability because survival of Neotyphodium fungi in seed of tall fescue stored for 8 yr at 4 [degrees] C and -196 [degrees] C has been excellent (Clement, unpublished data, 1998).
Abbreviations: PCR, polymerase chain reaction; PDA, potato dextrose agar; SEM, scanning electron microscope; WRPIS, Western Regional Plant Introduction Station.
Three accessions of annual ryegrass--a diploid, self-incompatible, and wind pollinated species--were identified in the WRPIS collection with sufficient original seed remaining for regeneration.
In addition, plants of current WRPIS distribution stocks of the three accessions were grown in the greenhouse and isozyme analysis completed.