The WSLH laboratory-based CRE surveillance program requests all clinical microbiology laboratories to submit carbapenem-nonsusceptible Enterobacteriaceae isolates to WSLH for PCR testing to determine the presence of genes encoding carbapenemase, including KPC, NDM, IMP, VIM, and OXA-48.
During February-May 2015, a total of 49 CRE isolates from 46 patients that met the National Healthcare Safety Network case definition for laboratory-identified CRE events (2) were submitted to WSLH (Figure 1).
WSLH standard operating procedure (SOP) for both the nitrate + nitrite and fluoride analysis followed ESS Inorganic Method 220.
The presence or absence of total coliform bacteria was analyzed as per WSLH ESS Water Micro Method 300, which is a chromogenic substrate method approved by U.
To achieve sufficient statistical power from a large number of PT challenges over the study period, phase 1 included 22 certified clinical laboratories participating in both the NYSDH and WSLH PT programs for BPb.
In phase 1, clinical laboratories were selected for a blinded assessment of laboratory performance in the following manner: A list of laboratories that participate in the NYSDH BPb PT program was matched with one from the WSLH BPb PT program.
Thirty-eight PUFs were sent to the WSLH
and analyzed for 23 congeners (the same as those analyzed in blood serum, less BDE-119).
All blood collection containers used in the program were lot tested for the presence of lead by a WSLH adaptation of published protocols (20).
Participants then reported FP results to WSLH for evaluation.
The WSLH is attached to the University of Wisconsin-Madison, and many of our senior scientists are faculty and academic staff at the university.
The WSLH PT program was originally set up to send out three unknowns monthly.
For comparison, WSLH
repeated the rapid assay tests of the specimens using Becton, Dickinson, and Company ColorPAC(TM) Cryptosporidium/Giardia rapid assay from the same lot used at the southeastern Wisconsin laboratory.