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References in periodicals archive ?
To further validate these findings by qRT-PCR, we analyzed the expression of the same five TTSa-RNAs mentioned above by qRT-PCR in whole cell extracts in HCT116 [Dicer.sup.EX5] cells as compared to parental cells.
Whole cell extracts were separated by SDS-PAGE and analyzed by Western Blot for FKBP51 (la) or FKBP52 (lb) and beta actin as a loading control.
Thus, the use of whole cell extracts, which contain several components having different possible intracellular targets, may provide an advantage over using one isolated plant compound.
These cell lines were then used to study phenotypical changes affected by the mutations, including DNA repair by whole cell extracts and the accumulation of oxidative DNA damage in genomic DNA of cells after exposure to oxidative stress.
Unfortunately, we could not perform a meaningful enzyme assay in whole cell extracts because cell sonication generates nicked nuclear DNA.
Cells were exposed to 40 [micro]M betanin for various time points (0, 4, 8, 12, 24 h) and whole cell extracts were prepared based on the method of Pardhasaradhi et al.
This enabled us to compare the relative levels of activity in both cytoplasmic and whole cell extracts from different stages of development.