p53 helps with this balancing act in the female embryo by producing normal levels of Xist RNA, part of an intricate molecular process important for X chromosome inactivation
. This, in turn, leads to healthy neural tube development.
Brown, "A skewed view of X chromosome inactivation
," Journal of Clinical Investigation, vol.
Belmont, "Methylation of HpaII and HhaI sites near the polymorphic CAG repeat in the human androgenreceptor gene correlates with X chromosome inactivation
," American Journal of Human Genetics, vol.
Regulation of X chromosome inactivation
in development in mice and humans.
Since each stem cell located in the basal layer of epidermis or hair follicle would give rise to a clone of progeny cells that would populate an intraepidermal column, we reasoned that the width of this clonally derived column could be determined by obtaining samples of increasing diameter and examining the DNA for the presence of a maternal versus paternal pattern of X chromosome inactivation
. We assumed that if our biopsy contained only a single clone of cells, a clear-cut maternally or paternally derived banding pattern would be present, but if more than 1 clone was present, a mixed pattern of maternal/paternal banding patterns would emerge.
Tissue specificity of X chromosome inactivation
"There is recent evidence that this x chromosome inactivation
occurs early during development," says Dr.
Topics include DNA methylation and the mammalian genome, methods for the genome-wide and genome-specific analysis of DNA methylation levels and patterns, histone modifications, histone variants and nucleosome occupancy, molecular mechanisms of polycomb silencing, non-coding RNAs in gene regulation, plant epigenetics using a comparative approach, embryonic stem cell epigenetics, the biology of genomic imprinting, X chromosome inactivation
, cancer epigenetics, the role of micro DNAs in human cancer, the impact of the environment on epigenetic regulation of gene expression and developmental programming, and trans-generational epigenetic inheritance.
Eight chapters deal with specific common molecular genetic diseases: muscular dystrophy, unstable trinucleotide repeats, familial adenomatous polyposis, cystic fibrosis, congenital adrenal hyperplasia, hemoglobinopathies, Prader Willi and Angelman syndromes, and analysis of X chromosome inactivation
. Each chapter contains a brief introduction, strategies for analysis, methods and materials, and multiple examples, interpretations, notes, and references.