The cytotoxicity of NSS oil was evaluated using the XTT
cell proliferation Kit II according to the method of Zheng et al (2001).
The cytotoxic effect of ISO in HT-29 cells and the concentration of ISO that decreased the cell viability by 50% ([IC.sub.50]) were determined using trypan blue dye exclusion test and XTT
assay based on the kit protocol.
) reduction assay is based on the reduction of XTT
tetrazolium formazan by active mitochondria in Candida cells (28).
The cytotoxic effects of gemcitabine and combination of gemcitabine and FA were determined using the XTT
Hydrosoluble formazan XTT
: its application to natural products drug discovery for Leishmania.
The multiparametric cytotoxicity assay kit used allows the evaluation of the material toxic effects in the same cells through three different parameters: mitochondrial metabolism and respiratory toxicity (XTT
); lysosomal integrity and membrane permeability (NR); and cell proliferation and presence of DNA (CVDE).
Viability was measured by XTT
assay (a) and the levels of TGF-[beta]1 (b) and [PGE.sub.2] (c) in culture medium were measured by ELISA.
Quantitative (Colony Count Method and XTT
Assay) Assessment of Biofilms.
Cerca, "Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT
colorimetry," Journal of Antimicrobial Chemotherapy, vol.
The viability of macrophages was determined using the Cytotoxicity Detection Kit (Roche) based on the measurement of lactate dehydrogenase (LDH) released from damaged cells and the XTT
cell proliferation assay (Life Technologies) based on a conversion of the tetrazolium dye XTT
to reduce colored form by transplasma membrane electron transport system.
Cell viability was measured with the help of a cell proliferation assay kit (Welgene Inc., Korea), using 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5-carboxanilide (XTT
###homogenized in a###antibacterial agent###observed using XTT