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XXOXanthine/Xanthine Oxidase
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Furthermore, we measured the ROS generation in cells treated with xanthine/xanthine oxidase as positive control and we found an increase of 26.20% [+ or -] 4.39 of DHEt positive cells (Figure 1, Suppl.
The xanthine/xanthine oxidase treatment was used as a positive control.
[O.sub.2.sup.-] generation in the xanthine/xanthine oxidase system
The effect of each component of eviprostat on [O.sub.2.sup.-] production was investigated in a cell-free xanthine/xanthine oxidase [O.sub.2.sup.-]-generating system (Storch and Ferber, 1988; Kyoi et al., 2004).
In order to determine whether these molecules (2-6) possess a direct superoxide scavenging activity [O.sub.2.sup.*-] formation induced by xanthine/xanthine oxidase system was measured.
5 <0.05 <2 6 <0.05 <2 First row shows the results in xanthine/xanthine oxidase and second row shows the results in AHP/xanthine oxidase system.
In the xanthine/xanthine oxidase system the extract and fractions were effective in both inhibition of uric acid formation and inhibition of NBT reduction by [O.sub.2.sup.-*].
In the xanthine/xanthine oxidase system, EAF was more effective at both inhibiting xanthine oxidase activity and inhibiting NBT reduction.
It was shown that it was only the silybin (1) that inhibited uric acid formation in xanthine/xanthine oxidase system with an I[C.sub.50] of 32.2 [micro]M and prevented LDL from hem-mediated oxidative degradation.
Superoxide anion, generated by the xanthine/xanthine oxidase system, converted nitroblue tetrazolium to formazan that had maximum absorption at 560 nm.
wrightii for superoxide anion ([O.sup.-sub.2]) generated by xanthine/xanthine oxidase and hydroxyl radical (.OH) by Fenton reaction ([FeSO.sub.4]/[H.sub.2][O.sub.2]) was determined by an electron spin resonance (ESR) spectrometer (JESFR3O, JEOL, Tokyo, Japan), as described previously (Myagmar and Aniya, 2000).
wrightii showed very strong scavenging action for superoxide anion generated from xanthine/xanthine oxidase system ([IC.sub.50] = 0.85 [micro]g/ml) and also markedly inhibited CL production from PMA-stimulated leukocytes (7.5 [micro]g/ml).