For in vitro transcription, full-length and truncated Egr3 construct containing Xpress tag at the N-terminus and DsRed2 at the C-terminus were amplified by PCR and cloned into BamHI and HindIII site of pRSET-A vector (Invitrogen, USA).
Anti-Xpress antibody detecting Xpress tag on the fusion construct (Figure 1B) produced a signal much closer to Egr3-DsRed2 live imaging (Figure 5), although the pattern was more diffused than that of DsRed2 live imaging.
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