Previous understanding of ZFY
gene role as Testes Determining Factor (TDF) has now been strengthened by many experimentations.
The mean read depth ratio of ZFY to ZFX ranged from 0.
Fractional cffDNA concentration estimated by calculating the mean read depth of ZFX and ZFY ranged from 5.
Las exitosas amplificaciones de los segmentos individuales ZFX y ZFY
en PCR simples, asi como la deteccion conjunta de ambos segmentos ZFX y ZFY
en PCR multiple se detallan en el Cuadro 5.
1996), "Estimating the Age of the Common Ancestor of Men from the ZFY
Intron," Science 272: 1357-1365.
Multiplex 1: SRY, sY84, sY134, sY255; Multiplex 2: ZFY
, sY86, sY127, sY254; Multiplex 3: SRY, sY145, sY153; Multiplex 4: ZFY
, sY81, sY121; and Multiplex 5: SRY, sY124, sY242, sY239.
5] Human genes: HBB, hemoglobin, beta; RHD, Rh blood group, D antigen; SRY, sex determining region Y; CCR5, chemokine (C-C motif) receptor 5 (gene/ pseudogene); ZCCHC2, zinc finger, CCHC domain containing 2; ZFX, zinc finger protein, X-linked; ZFY
, zinc finger protein, Y-linked.
Este enunciado ha sido sugerido luego del analisis comparativo de los genes ZFX y ZFY
bovino, porcino y equino realizada por Poloumienko (2004) y la amplificacion del gen ZF en humanos y diversas especies de animales domesticos y silvestres realizados por Pomp et al.
Several protocols have been established for sexing embryos in farm animals, such as karyotyping (King, 1984), H-Y antigen detection (Andersib, 1987), X-linked enzymatic determination (Monk and Handyside, 1988), and based on the identification of the Y chromosome, such as SRY, ZFY
and TSPY genes, include in situ hybridization, Southern dot blotting, polymerase chain reaction (PCR), Loop-Mediated Isothermal Amplification (LAMP) (Miller, 1991; Bredbacka and Peippo, 1992; Gutierrez-Adan et al.
The loci for an EGG comparison were HLCS on chromosome 21 and ZFY
(zinc finger protein, Y-linked) on chromosome Y from pregnancies with a male fetus.
Interestingly, we observed the highest detection rate for hCG[beta] mRNA during the first trimester of pregnancy, whereas Lo's group found the lowest detection rate for ZFY
mRNA in early pregnancy (8).
1, RT-PCR products corresponding to ZFY
mRNA were observed only when male placental total RNA was used in the RT-PCR assay (Fig.