In preclinical studies, the TKM-Ebola therapeutic demonstrated 100 percent protection from an otherwise lethal dose of Zaire Ebola virus
when TKM-Ebola was used to treat previously infected non-human primates (Geisbert et al., The Lancet, Vol.
This new TKM-Ebola LNP formulation has demonstrated significant increases in potency in non-human primates infected with the Zaire Ebola virus. At 0.5 mg/kg, 100% of the infected animals survived after receiving TKM- Ebola daily for seven days.
Importantly, the lyophilized LNP formulation also provided 100% survival in non-human primates infected with the Zaire Ebola virus with no loss in potency at 0.5 mg/kg dosed daily for seven days.
During the EVD outbreaks of 2003 in Gabon and the Congo, Zaire Ebola virus RNA and antibodies were detected in live-caught specimens from 3 fruit bat species (Epomops franqueti, Hypsignathus monstrosus, Myonycteris torquata); virus sequences were found in the livers or spleens of a few bats (6).
For bat species from Cameroon and Guinea, we screened for Zaire Ebola virus RNA by seminested reverse transcription PCR (RT-PCR) targeting the nucleoprotein region of the virus genome.
We observed Zaire and Sudan Ebola virus seropositivity in these 3 species with almost all cutoff methods: 0.2%-3.3% for Zaire Ebola virus and 1.0%-2.9% for Sudan Ebola virus in E.
In a paper published for the journal (http://embomolmed.embopress.org/content/early/2014/12/29/emmm.201404792) EMBO Molecular Medicine , scientists have deduced that the current outbreak of the fatal disease, caused by the Zaire Ebola virus
or EBOV, was triggered by a young boy who got Ebola from a hollowed tree in their community.
Difficulties of interpretation of Zaire Ebola virus
PCR results and implication in the field.
The family Filoviridae contains 2 genera: Marburgvirus, which contains Marburg virus (MARV), and Ebolavirus, which contains 4 viruses: Zaire Ebola virus (ZEBOV), Sudan Ebola virus, Reston Ebola virus (REBOV), and Cote d'Ivoire Ebola virus, and 2 tentative species (Bundibugyo Ebola virus and Lloviu Ebola virus) (2,3).
OD, optical density; R + Z, ELISA using a 1:1 mixture of recombinant nucleoproteins of Reston and Zaire Ebola viruses; R, Reston Ebola virus ELISA; Z, Zaire Ebola virus ELISA; ND, not determined; A, adult; -, negative; ++, strongly positive; NT, not tested; +, positive.
gambianus 0.63 - - Negative control (RAB691/dO) 0.27 1.42 + + Positive control (RAB691/EBOV-N) * ZEBOV, Zaire Ebola virus
; REBOV, Reston Ebola virus; OD, optical density; A, adult; SI, sexually immature.
The Zaire Ebola virus
species (Ebola virus-Z), 1 of the 4 known species of Ebola virus, occurs in central Africa and kills 80% of infected persons within a few days (3,4).