A sterile metal spike was used to inoculate part of the specimen (minimum of 10 inocula) onto the nutrient agar (a Sabouraud medium without
cycloheximide, Becton-Dickinson, Franklin Lake, US), that was incubated at room temperature for 14 days.
To measure rates of protein synthesis in soleus and EDL, L-[U-[sup.14]C]-tyrosine (0.05 [micro]Ci/mL) was added in Krebs-Ringer bicarbonate buffer without
cycloheximide only after the pre-incubation.
To estimate the half-life of the protein, cells transiently expressing RT1.14oil, or RT1.14opt-in, were after two days treated with
cycloheximide and lysed 0,2,4, and 6 hours after the on-start of the treatment.
25ml of the prepared SDA was poured in each Petri-dishe and supplemented with (5[micro]g/ml)
cycloheximide and (10 [micro]g/ml) amoxicillin for maintenance and growth of dermatophyte isolate.
Cycloheximide and lipopolysaccharide downregulate aENaC mRNA via different mechanisms in alveolar epithelial cells.
Senescence and postharvest performance of cut Nerine sarniensis flowers effects of
cycloheximide. Int.
To test this, we performed qPCR and
cycloheximide (CHX) treatment and immunoblot analyses, respectively.
cycloheximide [1 [micro]M]), was studied in the MG-63 osteoblast-like cells.
The scales of skin scraped from lesions and epilated hair were inoculated on Sabouraud Dextrose Agar (SDA) with Chloromycetin and
Cycloheximide by spot inoculation method for isolation of fungi.