A: gnatopodo 1 (Gn1), meroquelado, Aora typica; B: gnatopodo 2 (
Gn2) carpoquelado, Ericthonius punctatus; C, gnatopodo 1 (Gn1) subquelado, Stenothoe penelopae; D, gnatopodo 1(Gn1) transverso, gnatopodo 2 (
Gn2) quelado, Americhelidium setosus (figuras modificadas basados en: Barnard & Karaman 1991, Bousfield & Chevrier 1996, Krapp-Schickel 2006).
GN1 and
GN2 scaffolds materials were analyzed in vertical and transversal cross sections by scanning electron microscopy (Zeiss EVO 40) in low-vacuum modality and by applying a voltage of 25 kV.
In carbon assimilation test the isolate
GN2 grew poorly in the presence of sucrose and fructose as a sole carbon source and formed abundant mycelium on the media with glucose and maltose, whereas in nitrogen utilization test, it has grown abundantly on glutamic acid and poorly on histidine, methionine, and leucine.
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coli by using the Biolog
GN2 panel (Biolog, Inc., Hayward, CA, USA) (100%, T = 0,534), after incubation of the panel in an atmosphere containing 6% C[O.sub.2] for 1 day.
Biolog
GN2 96 Well Microplates (Hayward, California) were inoculated with the prepared inocula and incubated for 24 hours at 37[degrees]C.
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11) was adequate to identify spikes corresponding to two of the giant neurons, which we label GN1 and
GN2 following the convention of Igelmund & Wendler (1991a).
Type A and type B isolates were differentiated by biochemical subtyping (glycerol fermentation) with the 96-well automated MicroLog MicroStation System with
GN2 Microplates (Biolog Inc, Hayward, CA, USA) (13).
For biochemical subtyping, the 96-well automated MicroLog MicroStation System with
GN2 Microplates (Biolog Inc, Hayward, CA) was used.