Compared to FGFR gene mutation and translocation, gene amplification
of FGFR is most well studied.
1, the structure of the differential amplification
micro actuator was primarily composed of GMA and a displacement amplification
There is another chemistry-based contamination control approach which is available in most amplification
methods and has completely general utility; it allows for the uniform destruction of any and all carryover, contaminating amplicon in a reaction before it can cause a problem, and it does so in a simple, elegant manner.
was assessed basing on analytical methods assuming the specific geological cross-section and therefore calculated amplification
had little precision.
Investigations are ongoing to determine the nature of this amplification
observed in the absence of trigger, and our data suggest that unconventional DNA synthesis may be involved (unpublished data).
In accordance with the manual, a whole genome amplification
system (WGA; Sigma-Aldrich, Saint Louis, MO, USA), which was developed for amplification
of genomic DNA, was used to amplify viral double-stranded cDNA.
Ulla Deutsch, Senior Global Product Manager Amplification
The RD-100i analyzer, which is based on the company's proprietary gene amplification
method OSNA (One-step Nucleic Acid Amplification
), can determine the status of lymph-node metastasis of breast cancer in about 30 minutes during an operation.
Unlike PCR, MDA allows nonspecific amplification
of the complete genome, and, as a whole genome amplification
technique, can be used to pretreat samples that are later used for PCR.
To address the hypothesis that all medulloblastomas with oncogenic amplification
exhibit either the "anaplastic" phenotype or the large cell phenotype, medulloblastomas exhibiting oncogene amplification
were graded for anaplastic features by a separate neuropathologist (P.
To increase the sensitivity, we also tested two-step and three-step amplification
Among the 7,220 male samples analyzed, the X chromosome-specific allele at the Amelogenin locus was not amplified in three samples using the AmpFLSTR[R] Profiler Plus[TM] amplification