t] of 24 hours with bromophenol blue
, that probably indicate the existence of preferential paths inside the reactor.
Loaded 5[micro]l of PCR product with 4 [micro]l bromophenol blue
(Loading Dye) in 1.
A) Submitted to the bromophenol blue
technique; (B) Staining with xylidine ponceau; (C) Submitted to the PAS the technique; (D) Submitted to the von Kossa method (Calcium in brown color).
(BPB) is regularly used as a dye marker to find out an ion front in the electrophoretic techniques.
In the histochemical characterization of EFNs, a positive reaction of ferric chloride (Figure 2b), zinc chloride iodine (Figure 2c), bromophenol blue
(Figure 2d), Periodic Acid Schiff (Figure 2e) and Fehling (Figure 2f) revealed the presence of phenolic compounds, starch, proteins, neutral polysaccharides and reducing sugars, respectively.
TEMED (Merck) Bromophenol blue
(Merck) Preparation of Gel:
4]), ethylene triamine tetra acetic acid (EDTA), agarose, RNase, ethanol, bromophenol blue
, ethidium bromide, physiological saline, DNeasy blood and tissue Mini Kit (Qiagen, Hilden, Germany) and High sensitive 8-OHdG check ELISA kit (Japan Institute for the Control of Aging, Fukuroi, Japan or Genox Corp.
Bovine serum albumin (BSA), bromophenol blue
, 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), eosin, glycerol, hematoxylin, hex-adecyltrimethylammonium bromide (HTAB), 2-mercaptoethanol, naphthylenediamide dihydrochloride, nicotinamide adenine dinucleotide phosphate (NADPH), oxidized glutathione, phosphoric acid, reduced glutathione, sodium acetate, sodium dodecyl sulfate (SDS), sodium nitrite, sulfanilamide, 3,3',5,5'-tetramethylbenzidine (TMB), triethanolamine, Tris-HCI, Tween 20, and 2-vinylpyridine were purchased from Sigma Chemical (St.
emits a strong fluorescent signal; either omit it from the loading buffer, run the bromophenol blue
dye front off the polyacrylamide gel or cut off the dye front before transfer.
Electrophoresis was run at 125 V constant voltage for 180 min and the run stopped after the bromophenol blue
dye reached the end of the plate.
8, glycerol, deionised water, sodium dodecyl Sulphate, bromophenol blue
Congo red has been used in colorimetric determinations of chitosan and poly (N-vinyl-2-pyrrolidone) while bromophenol blue
has been used in colorimetric assays for antimalarial drugs (4-6).