The observed or actual bp length of the PCR cDNA
should correspond to the predicted bp length, as calculated from the number of base sequences flanked by the primers.
In addition, there was a feeling that CDNA
did not have a clearly defined purpose.
Within the United States, the Department of Energy funds cDNA
sequencing at about half a dozen universities, says Daniel Drell, a biologist at the agency.
Transformed Escherichia coli harboring pET-28a/ISG15 cDNA
was sub-cultured in Lurria Bertani media supplemented with Kanamycin (100 [micro]g/mL) and incubated at 37[degrees]C until the OD600 reached 0.
To verify the reliability of cDNA
RDA results, the abundantly expressed gene RpL7 was selected to detect the mRNA level between the deltamethrinresistant samples and the controls using real-time fluorescent quantitative PCR.
Briefly, recombinant pMD-18T with SPLUNC1 ORF was digested with SnaB I and Not I and the SPLUNC1 cDNA
fragment was inserted into pPIC9K vector previously linearized with similar enzymes to obtain the pPIC9K/SPLUNC1 cDNA
Two micrograms of total RNA was used for first strand cDNA
synthesis using a PrimeScriptTM RT Reagent Kit (TaKaRa).
In this paper we report the construction of a normalized full-length enriched cDNA
library and primary analysis of 365 ESTs from female Siberian tiger fibroblast cells.
Prior to cDNA
synthesis, RNA concentration was determined by UV spectrophotometry and integrity of extracted RNA was checked by agarose gel electrophoresis.
Isolation of cDNA
was performed by 3' and 5' full RACE Core Set Ver.
Horizon will also be making available over 125 off-the-shelf RNAiONE validated shRNAs and over 70 cDNA
17 February 2014 - UK translational genomics company Horizon Discovery said Monday it had become a distributor of German Sirion Biotech GmbH's RNAiONE custom shRNA development services and off-the-shelf validated shRNA and cDNA