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Khan and Husain (2007) observed the same optimum dose (40 mg/l) of crystal violet sould be decolorized by bitter gourd peroxidase.
This is an interesting fact since, to the author's knowledge; triphenyl-methane dyes such as crystal violet in dilution has not been reported high decolorization rates through direct photolysis.
In gram-positive cells, crystal violet slowly drains from the thick peptidoglycan barrier, but not quickly enough to leave the cell colorless during the protocol.
For quantitation of the CFUs formed by MSCs, crystal violet was extracted and normalized to the number of CFUs in each sample.
05% crystal violet (CV) solution in 20% ethanol was added and cells were allowed to stain for 30 minutes.
Thin smears were prepared by mixing equal amount of undiluted carbol fuchsin, malachite green, methylene blue, and crystal violet solution with concentrated faecal sample on a glass slide.
The aim of this work was to evaluate the efficiency of synthesized zeolites from Brazilian coal fly and bottom ashes as adsorbent in the removal of basic dye crystal violet from aqueous solutions.
The second method of alteration in outer membrane permeability was detected by crystal violet assay (Vaara and Vaara 1981).
All test strains formed biofilms as assessed with the crystal violet assay.
In this assay, the more biofilm formed on the well, the greater the amount of staining with crystal violet (and the more intense the color will be; Figure 3).
Thereafter, cells were fixed and stained for quantification of relative cell number (by crystal violet staining) or for the interendothelial contact molecule CD31 and f-actin.