The enhancing effect of luteolin at 5 [micro]M on ALP activity in MG-63 cells was blocked completely by the presence of cycloheximide
, an inhibitor of protein synthesis.
mice and treated with apoptosis inducers cycloheximide
(CHX) (1 [micro]g/mL; 10 [micro]g/mL; 30 [micro]g/mL) and puromycin (PM) (1 [micro]g/mL; 10 [micro]g/mL; 30 [micro]g/mL), respectively, to validate their inability to undergo apoptosis.
The sediment of specimens was inoculated on Sabouraud dextrose agar, Sabouraud dextrose agar (Merck, Germany) with cycloheximide
(Sigma-Aldrich, USA), paraffin agar (0.
Subsequently couplets were washed three times and cultured in PZM3 with 10 g/ml of CB and 10 g/ml of cycloheximide
1993) Differential action of cycloheximide
and activation stimuli on transcription of tumor necrosis factor-alpha, IL-1 beta, IL-8, and P53 genes in human monocytes.
Mycosel is a highly selective media, which contains cycloheximide
to suppress the growth of many fungi; however, the agents of endemic mycosis, dermatophytes, and Candida albicans are resistant to cycloheximide
and will grow.
It is germ tube test negative, hydrolyzes urea, produces melanin, and does not grow on cycloheximide
Chlamydia trachomatis was identified in McCoy cell culture treated by cycloheximide
and genital mycoplasmas (Ureaplasma urealyticum and Mycoplasma hominis) were determined according to their biochemical properties in liquid selective medium from the swab of the cervix, urethra and prostatic exprimate.
Samples from suspected areas were examined directly with 15% potassium hydroxide (KOH) and cultured on Sabouraud dextrose agar (SDA) and SDA-containing chloramphenicol and cycloheximide
, and SDA-containing only chloramphenicol.
Mycological analysis was performed by direct examination and culture in Sabouraud medium with chloramphenicol and cycloheximide
The initial comparison of protocols used by participating laboratories revealed that cycloheximide
or puromycin was sometimes used for NMD treatment, with incubation times between 4 and 8 h and concentrations between 100 and 250 [micro]g/mL, the use of 8 cDNA synthesis kits, 12 different DNA polymerases, and transcript isolation strategies that included band excision, subcloning, and sequencing.
Synchronization of porcine oocyte meiosis using cycloheximide
and its application to the study of regulation by cumulus cells.