dGTP


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Related to dGTP: dTTP, dATP
AcronymDefinition
dGTPDeoxyguanosine Triphosphate
dGTPDirector General Telecommunications Policy Branch (Canada)
dGTPDairyland Garden Tractor Pullers (Wisconsin)
dGTPDansk Garden Tractor Pulling (Denmark)
References in periodicals archive ?
Al Rifai added that DGTP would detail 'no cost' and 'low cost' options for hoteliers seeking to apply sustainability solutions for 'quick wins'.
However, each of these reaction mixtures contained 20 nM of the template, 10 nM of each primer 5' -TATTGATTGTGAATTA(C/G)-3', and 100 [micro]M of a single dNTP (dCTP, dGTP, dATP, or dTTP).
For codons 12, 13, and 14 of wild-type KRAS and 12b KRAS mutant (G [right arrow] A), both molecules are elongated when dGTP is dispensed, but the nascent strands are out of phase because the strand replicating the wild-type allele incorporates 2 dGTPs, whereas the one replicating the mutant allele only incorporates one dGTP (Figures 5, A, and 7, A).
As previously described [28] PCR was performed in a final reaction volume of 100 [micro]L with 5 [micro]L of sample material and 95 [micro]L of kit working master mix (containing Mg[Cl.sub.2], KCl, AmpliTaq, gold DNA polymerase (AmpliTaq; Perkin-Elmer; Foster City, CA), uracil-N-glycosylase, dATP, dCTP, dGTP, dUTP, dTTP, and biotinylated PGMY primers and [beta]-globin primers GH2O and PCO4).
The PCR reaction mixture consisted of 1200 nM of each primer, 200 nM probe, 0.4 U AmpliTaq gold polymerase, 200 nM each of dATP, dCTP, dGTP, and dTTP, 3.5 mM Mg[Cl.sub.2], and 1 x Taqman buffer A containing a reference dye.
100ng of DNA was dissolved in a 25[micro]l system that included 1.1 [micro]mol/LMg[Cl.sub.2], 200 [micro]mol/ LdNTP(dATP, dCTP, dGTP, dTTP), 1[micro]mol/L primers, and 1.5uTaq enzyme.
Polymerase chain reactions (PCRs) were performed for each individual in a 50-[micro]l total volume consisting of the following: 5[micro]l of DNA (10 ng/ml) from the individual; 1.5 [micro]l of a 10-mM solution of each primer (P-2 and P-8; Griffiths et al., 2002); 5[micro]l of a dNTP mixture containing 1 mM each of dATP, dCTP, dGTP, and dTTP; 2[micro]l of a 25-mM solution of magnesium chloride; 0.2 [micro]l of Taq DNA polymerase (Promega: GoTaq Flexi DNA Polymerase; Promega Corp., Madison, Wisconsin); 10 [micro]l of a 5X reaction buffer (Promega); 24.8 [micro]l of sterile water.
A 2 [micro]L volume of DNA template was added to 23 [micro]L of reaction mixture, which contained 2.5 [micro]L of PCR buffer 10x (10 mM Tris HCl, 25 mM KCl), 1mM of Mg[Cl.sub.2], 0.5 [micro]M of each primer, 100 [micro]M of each deoxynucleotide trifosfate (dATP, dGTP, dCTP, dTTP) and 1.5U of Taq polimerase (Invitrogen--Sao Paulo, Brazil).
Labeling was obtained using a PCR procedure with the following dNTP concentrations: 200 [micro]M dATP, 200 [micor]M dCTP, 200 [micro]M dGTP, 150 [micro]M dTTP, and 50 [micro]M dig-11-dUTP (Roche Molecular Biochemicals).
Condicoes de amplificacao do DNA: Preparou-se uma mistura de reagentes em um microtubo para capacidade para 200 [micro]L que consistiu em 1, 5x de tampao Taq, 4 mM de Mg[Cl.sub.2], 0, 2 [micro]M de cada oligonucleotideo (G1 e G2), 100 [micro]M de dNTP (dATP, dTTP, dCTP e dGTP) e 2,5U, de Taq DNA Polimerase (Ludiwing [R]).
Polymerase Chain Reactions (PCR) were carried out in a final volume of 25 [micro]l containing 20 ng template DNA, 100[micro]M each dATP, dTTP, dGTP & dCTP, 20 ng of decanucleotide primers, 1.5mM Mg[Cl.sub.2], 1 x taq buffer (Fermantas) [10mM Tris-HCl (pH 9.0), 50mM KCl, 0.01% gelatin] and 0.5 U Taq DNA polymerase (Fermentas).