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dUTP2'-Deoxyuridine 5'-Triphosphate
dUTPDhaka Urban Transport Project (World Bank; Bangladesh)
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Apoptosis was evaluated by using terminal deoxynucleotydil transferase dUTP nick and labelling (TUNEL) method.
a-c) Values from three replicates with different superscripts denote a significant difference relative to other groups (p<0,05), TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; DAPI, 4',6-diamidino-2-phenylindole.
Keywords: Behcet disease, neuro-Behcet disease, immunoglobulin G, terminal deoxynucleotidyl transferase dUTP nick end labeling, neurotoxicity
DAPI, 4,'6-diamidino-2- phenylindole; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; FITC, fluorescein isothiocyanate; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling.
As formulated into most premade reaction kits, application of ung contamination control therefore consists of nothing more than adding the ung to dUTP containing master mix (if not already present), and adding a few-minute PCR reaction preincubation step at moderate temperatures (usually 37[degrees]-45[degrees]C).
Una aplicacion relevante de implementacion de un sistema de DUTP en Chile lo constituyen las Areas de Manejo y Explotacion de Recursos Bentonicos (AMERB).
Abbreviations: CFL, compact fluorescent lamp; ERG, electroretinography; H&E, hematoxylin and eosin staining; IHC, immunohistochemistry; ROS, reactive oxygen species; TEM, transmission electron microscopy; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.
10) Several techniques were described to assess the DNA integrity of sperm, and these techniques include TdT (terminal deoxyribonucleotidyl transferase)-mediated dUTP nick-end labeling (TUNEL) assay, aniline blue straining.
Detection of Chromatin and DNA Damage: The common methods to detect sperm chromatin and DNA damage are sperm chromatin structure assay (SCSA), sperm chromatin dispersion (SCD) test, Comet Assay, terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and fluorescence in situ hybridization (FISH).
Next the embryos were incubated in fluorescein-conjugated dUTP and TdT (TUNEL reagent; In situ Cell Detection kit, Roche Diagnostic, Germany) for 1 h at 38[degrees]C and 5% C[O.
A terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay (Promega) was performed according to the manufacturer's instructions.
Sections 20 mm in thickness were obtained and stained with (H&E) and with TdT-mediated dUTP biotin nick-end labelling (TUNEL).