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Specifically, genes were compared to the dbGaP. The five categories most significantly associated with differentially methylated genes in maternal blood, i.e., those with the smallest adjusted p-value after applying the Benjamini-Hochberg method for multiple testing correction were BMI (p = 2.0 x [10.sup.-5]), blood pressure (p = 3.8 x [10.sup.-5]), body weight (p = 0.0014), albumins (p = 0.0051), and hematocrit (0.0201) (Table 4).
Since several genome-wide association studies were conducted to determine the genetic risk factors for blood pressure [16-20], we further investigated the relationship using the Phenotype-Genotype Integrator in the NCBI dbGaP website [38-40].
The datasets used for the analyses described in this manuscript were obtained from dbGaP at http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gap through dbGaP accession numbers phs000092.v1.p1 and phs000125.v1.p1.
John Heit and his research team at the Mayo Clinic in Minnesota for the use of their data and assistance in securing the data needed for this project from the database Genotypes and Phenotypes (dbGap) archived at the National Center for Biotechnology Information (NCBI), U.
dbGAP will also provide pre-computed analyses of the level of statistical association between genes and selected phenotypes.
National Library of Medicine (NLM), "NIH Launches dbGaP, a Database of Whole Genome Association Studies" December 12, 2006
The discovery dataset incorporated a total of 5905 subjects from five GWAS, of which three studies were "in-house" studies: (1) Omaha Osteoporosis Study (Caucasian ancestry, n = 987), (2) Kansas City Osteoporosis Study (Caucasian ancestry, n = 2250), and (3) China Osteoporosis Study (Han Chinese ancestry, n = 1547), and two studies were "external" studies obtained from the Database on Genotypes and Phenotypes (dbGaP): (1) Women's Health Initiative Observational Study African-American Substudy (African ancestry, n = 712) and (2) Women's Health Initiative Observational Study Hispanic Substudy (Hispanic ancestry, n = 409).
2009), with genotype data obtained using Illumina HumanHap550v3.0 chips (dbGaP Study Accession number: phs000093.v2.p2; see Supplemental Material, Table S1).
Replication was conducted among the MESA participants, whose data were obtained from dbGaP (database of Genotypes and Phenotypes, http://www.
Researchers can access the sequence data at dbGaP or the National Institute on Aging Genetics of Alzheimer's Disease Data Storage Site (NIAGADS), https://www.niagads.org
Phenotypes for multisubstance dependency and genome-wide SNP data from SAGE  were downloaded from dbGaP (http://www.ncbi.nlm.nih.gov/gap).
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