ddPCRDifferential-Display Polymerase Chain Reaction
ddPCRDouble Differential Polymerase Chain Reaction
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An even more recent evolution in PCR came in the form of partition-based PCR techniques, including ddPCR, which has enabled absolute quantification of DNA.
[2] Nonstandard abbreviations: HSCT, hematopoietic stem cell transplantation; ddPCR, digital droplet PCR; smMIP, single-molecule molecular inversion probe; UMID, unique molecular identifier; MRD, minimal residual disease; CEPH, Centre d'Etude du Polymorphism Humain.
Using ddPCR for the detection of DNA methylation in the TMPRSS4 promoter gene in bisulfite-converted samples, we were able to detect TMPRSS4 promoter hypomethylation with very low input of DNA, as low as 0.5 ng (3.3 copies/[micro]L; Figure 4).
The assay was described in the study, "Development of ddPCR blood-based diagnostic tests that simultaneously measure mRNA expression from immune and cancer cells." The GeneStrat test offers accurate, non-invasive blood-based mutation results with a 72-hour turnaround time for patients with non-small cell lung cancer, delivering rapid information for diagnosis or therapy monitoring to identify specific driver mutations with proven clinical utility.
The authors compared the performance of ddPCR with CastPCR and concluded that ddPCR was more robust and more sensitive than CastPCR.
To elucidate the molecular responses stimulated by excess copper exposure in Lemna gibba, genes with altered expression in the presence of copper were identified by ddPCR. Of the six genes identified, northern hybridization analysis demonstrated that four (callose synthase, HSP90, serine decarboxylase, and the biotin carboxylase subunit of acetyl-coenzyme A carboxylase) increased in expression, while the other two genes (the HAP5 subunit of the heme-activated protein (HAP) transcription factor and the chloroplast nucleoid DNA-binding protein CND41) decreased in expression.
Finally, we established a detection method to study DNA methylation of SFRP genes in MPM and normal plasma samples with droplet digital PCR (ddPCR).
Therefore, Oxnard spent years developing the ddPCR droplet digital polymerase chain reaction liquid biopsy.
The company's key technologies include droplet digital PCR (ddPCR) and next-generation sequencing (NGS).
Bio-Rad's QX100 Droplet Digital PCR (ddPCR) system has been used in a research setting for this purpose.