Results showed that in the beginning, the fluorescent emission of fluorescein isothiocyanate
molecule was turned off by modifying the surface of gold nanoparticles with fluorescein isothiocyanate
A, Flow cytometry results after the cells were treated with fluorescein isothiocyanate
(FITC) and propidium iodide (PI).
Bovine serum albumin, essentially fatty acid free (type A-6003), fluorescein isothiocyanate
, isomer I (type F-7250), and urea ultrapure (type U-0631) were purchased from Sigma-Aldrich, Inc., USA.
Microscopy Conditions for Fluorescence Imaging Using Confocal Microscopy Acquisition Channel Fluorophore Used in Excitation Laser This Study DAPI NA 405 nm FITC FITC 473 nm Texas Red Texas Red 559 nm Far red Alexa 633 635 nm Acquisition Channel Emission Image Display Color DAPI 425-525 Blue FITC 485-585 Green Texas Red 570-670 Red Far red 650-750 Red Abbreviations: DAPI, 4',6-diamidino-2-phenylindole;FITC, fluorescein isothiocyanate
; NA, not applicable.
The slides were washed three times with PBS-T (0.5% Tween 20 in PBS) for 5 min each, and then exposed to goat anti-mouse IgG conjugated with fluorescein isothiocyanate
(FITC) (1:100) (BOSTER, China).
RM 8640 consists of six plastic bottles each containing a 2 mL suspension of polymethyl methacrylate (PMMA) microspheres with a specified amount of immobilized fluorescein isothiocyanate
One set included GLP 13 (green) and GLP 21 (red), which were labeled by fluorescein isothiocyanate
and tetramethylrhodamine, respectively.
Quinine and harmine were non-covalently attached onto the surface of the starch nanoparticles, while gelatin nanoparticles were covalently labeled with fluorescein isothiocyanate
 Nonstandard abbreviations: FACS, fluorescence-activated cell sorting; AcNPV, Autographa californica nuclear polyhedrosis virus; LbL, layer-by-layer; PBS, phosphate-buffered saline; PAH, poly(allylamine hydrochloride); RITC, rhodamine isothiocyanate; mAb, monoclonal antibody; FITC, fluorescein isothiocyanate
; FCS, fetal calf serum; and geo MFI, geometric mean fluorescence intensity.
Oocysts were purified from the specimen (5) and stained with the Cryptosporidium spp.-specific antibody CRY104 labeled with fluorescein isothiocyanate
(Biotech Frontiers, North Ryde, Australia) for enumeration.
Biotin-labeled DNA was detected by use of avidin-conjugated fluorescein isothiocyanate
(FITC; Vector) and biotin-conjugated avidin (Vector).
The secondary antibodies were fluorescein isothiocyanate
(FITC)-conjugated swine anti-rabbit immunoglobulin (1:20; Dako, Glostrup, Denmark), FITC-conjugated rabbit anti-mouse immunoglobulin (1:20; Dako), Texas-Red-conjugated goat anti-mouse and goat anti-rabbit immunoglobulins (1:50; Molecular Probes, Leiden, Netherlands).