To evaluate the impact of the Macrorhabdus infection on the bacterial communities, culture-independent methods, by the use of amplicon-based sequencing as well as 16S rRNA-denaturing gradient gel electrophoresis
, were adopted.
To perform denaturing gradient gel electrophoresis
(DGGE) analysis, 16S rRNA gene fragments of the V3 region were amplified using the primers GC-clamp-341f (5'-TCC TAC GGG AGG CAG CAG-5') and 518r (5'-ATT ACC GCC GCT GCT GG-3') as described previously [10,11].
Tenders are invited for Supply and installation of denaturing gradient gel electrophoresis
Denaturing gradient gel electrophoresis
(DGGE) for microbial community analysis.
Application of Denaturing Gradient Gel Electrophoresis
(DGGE) to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other molecular techniques.
Abbreviations DGGE: Denaturing gradient gel electrophoresis
PGPB: Plant growth-promoting bacteria BNF: Biological nitrogen fixation.
Conformation sensitive gel electrophoresis for simple and accurate detection of mutations: Comparison with denaturing gradient gel electrophoresis
and nucleotide sequencing.
The denaturing gradient gel electrophoresis
(DGGE) is a well-established tool for molecular microbiology; this method makes possible the electrophoretic separation of DNA fragments on the basis of differences in nucleotide composition rather than their size (17,18).
[54.] Noda K, Kubota K, Yamasaki R (2000) Separation of lipooligosaccharides by linear gradient gel electrophoresis
. Anal Biochem 279: 18-22.
Gut microbiota composition was analyzed from fecal samples assessed at 1 and 6 months of age by quantitative polymerase chain reaction, 16S rRNA pyrosequencing, and denaturing gradient gel electrophoresis
In particular, the combination of a PCR-based assay of the variable 18S rRNA gene and amplicon separation using denaturing gradient gel electrophoresis
(DGGE) has been used to derive a much more complete picture of the soil fungal community than what has been achievable in the past .
* Denaturing gradient gel electrophoresis
(DGGE) system (e.g., CBS Scientific or BioRad Laboratories)