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(5-7) Various gonadotropin preparations are commercially available and used for COH such as human menopausal gonadotropin (hMG), human-derived follicle-stimulating hormone (hFSH), and recombinant FSH (rFSH).
Studies that compared hFSH with rFSH noted increased ovarian recruitment of follicles in the rFSH group.
Thus, the objective of the current study was to compare the efficacy of 4 different ovarian stimulation protocols, comprising hFSH, rFSH, hMG, and sequential use of hFSH and rFSH, on oocyte and embryo quality and IVF treatment outcome in patients undergoing IVF or intracytoplasmic sperm injection (ICSI).
Thereafter, ovarian stimulation was commenced for the study population as follows: group A: 40 patients who received hMG (Menogon[R], Ferring Pharmaceuticals A/S, Copenhagen, Denmark); group B: 40 patients who received hFSH (Fostimon[R], IBSA Institut Biochimique SA, Geneva, Switzerland); group C: 40 patients who received rFSH (Gonal-F[R], Merck, Serono, Rome, Italy); and group D: 40 patients who received hFSH (FostimonX[R], IBSA Institut Biochimique SA, Geneva, Switzerland) for the first 6 days, followed by rFSH (Gonal-F[R], Merck, Serono, Rome, Italy).
The dose-response relationships of p,p'-DDE were nonmonotonic for the cAMP accumulation induced by two different doses of hFSH, 0.03 IU/mL and 3 IU/mL.
(A) Chinese hamster ovary-FSH receptor (CHO-FSHR) cells were incubated with hFSH at 3 x [10.sup.-2] lU/mL and 3 IU/mL and increasing concentrations of p,p'-DDT were investigated (means [+ or -] SEM of four independent experiments performed in triplicate).
The maximum response to hFSH in the absence of p,p'-DDT was arbitrarily set at 100.
Chinese hamster ovary-follitropin receptor (CHO-FSHR) cells were stimulated with 3 x [10.sup.-2] IU/mL human FSH (hFSH) (left) and 3 IU/mL hFSH (right) in the presence of increasing doses of p,p'-DDE (A), o,p'-DDT (B), or BPA (C) (means [+ or -] SEM of three independent experiments performed in triplicate).
A gene encoding the [beta] subunit of hFSH (exon 1 and 2; GenBank accession no.
An expression vector for the hFSH gene (Figure 1) was constructed by inserting the goat [beta]-casein promoter (GenBank accession no.
Transformed cells and transgenic embryos were analyzed for transgene hFSH by PCR.
Introduction of the transgene hFSH into fetal fibroblasts
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