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We measured hK5, hK6, and hK11 by sandwich-type ELISA, 96-well plates, as described earlier (19-21) with use of biotinylated detection antibodies, alkaline phosphatase-conjugated streptavidin and diflunisal phosphate substrate.
In view of the encouraging results, the Board of Directors recommended the payment of an interim dividend of HK6 cents per share (2016 Interim: nil).
Preliminary studies have indicated that hK6 enhances the detection capability of the approved ovarian cancer assay CA-125," said Paul Baehr, IBEX President and CEO.
Serum hK2, hK3, hK5, hK6, hK7, hK8, hK10, and hK11 concentrations were significantly (P <0.
We found significant correlations between serum concentrations of hK2 and hK3 (positive) or hK10 (negative), between hK4 and hK13 or hK14 (both positive), between hK5 and hK6 (positive), and between hK7 and hK8, hK13, or hK14 (all positive).
In a recent study examining the serum of 100 ovarian cancer patients (80 with stage I/II disease), hK6 identified 42% more ovarian cancer patients than CA125.
She was the first to purify and functionally characterize hK6 and hK10 from ovarian cancer ascites fluid and hK11, a candidate prostate cancer biomarker, from seminal plasma.
Recently, four other members of the human kallikrein family, hK5, hK6 (neurosin), hK10 (normal epithelial cell specific 1), and hK11 (trypsin-like serine protease), were found to be potential serum biomarkers for breast, ovarian, and prostate cancer (10-13).
The Board has proposed an interim dividend of HK6 cents per ordinary share and a bonus issue of two bonus shares for every 10 existing ordinary shares held.
The human kallikrein 6 (KLK6) gene, which encodes for hK6 protein, was cloned independently by three groups.
More details about such procedures have been described elsewhere for similar immunoassays for hK6 (8) and hK10 (9).
Recently, three other members of this family, hK6 (also known as neurosin), hK10 [also known as normal epithelial cell specific 1 (NES1)], and hK11 [also known as trypsin-like serine protease (TLSP)], have been shown to be potential biomarkers for ovarian and prostate cancer (4-6).
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