hRXRHuman Retinoid X Receptors
Copyright 1988-2018, All rights reserved.
References in periodicals archive ?
For the reporter assay, plasmids expressing hRXR (10 ng), pGFP (10 ng), the relevant PPAR isoform (10 ng), and the corresponding reporter plasmid (30 ng) were cotransfected using FuGENE 6 transfection reagent (Roche) according to the manufacturer's instructions.
To detect changes in gene expression, L-02 cells were seeded into 6-well plates and cotransfected with plasmids expressing hRXR and different PPAR subtypes.
Human hepatic L-02 cells were cotransfected with plasmids expressing the three PPAR subtypes or an empty vector and hRXR. At 48 hours after transfection, cells were incubated with 1 [micro]M of each agonist or the vehicle control (0.1% (vol/vol) DMSO) for 24 hours.