hrSCFHuman Recombinant Stem Cell Factor
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In vivo produced blastocyst were cultured in porcine ES (PES) medium (50:50 mixture of Dulbecco's modified Eagle's medium (DMEM, low glucose) and Ham's F10 medium supplemented with 15% fetal bovine serum (FBS; collected and processed in Canada; Hyclone, Logan, UT, USA), 2 mM L-glutamine, 0.1 mM [beta]-mercaptoethanol, 1% MEM nonessential amino acids, 1% Antibiotic-Antimycotic containing cytokines, 40 ng/ml human recombinant SCF (hrSCF; R&D Systems, Minneapolis, MN, USA), 20 ng/ml human recombinant bFGF (hrbFGF; R&D Systems), and 10 ng/ml human recombinant LIF (hrLIF; Chemicon, Temecula, CA, USA).
As shown in Table 2, 197 IVF porcine embryos were seeded as whole explant and cultured in medium A (DMEM/F10 with 15% FBS), B (PES medium; DMEM/F10 with 15% FBS, hrSCF, hrbFGF, and hrLIF), C (DMEM/F10 with 15% FBS and hrbFGF), D (DMEM/F12 with 15% FBS), E (DMEM/F12 with 15% FBS and hrbFGF), or F (DMEM/F12 with 20% KSR and hrbFGF).