Conclusions: Our results suggest that compound 1 induces cytotoxic effects in human bladder cancer cells, including the cisplatin-resistant T24R2.
We further examined whether compound 1 could induce apoptosis in human bladder cancer cells.
Taken together, we suggest that compound 1 induces autophagy in human bladder cancer cells through the modulation of the AMPK/mTOR pathway.
In our system, compound 1 significantly led to the reduction of phospho-AKT levels, which in turn can induce a decrease of phospho-Bad, thus, inhibiting the anti-apoptotic proteins, Bcl-2 and BCL-XL Taken together, we concluded that compound 1 may induce the apoptosis of human bladder cancer cells through AKT inhibition, which leads to dephospho-Bad associated with an increase in active Bax.
From our data, compound 1 exerts a cytotoxic effect against human bladder cancer cells, including cisplatin-resistant cells, by promoting cell cycle arrest, apoptosis, and autophagic cell death.
Kazinol A induced cytotoxic effects against the human bladder cancer cells, T24 and T24R2.
papyrifera and determined the cytotoxic effects of each in the human bladder cancer cell T24 and its cisplatin-resistant derivative T24R2.
The human bladder cancer cell line T24 was obtained from the Shanghai Institute of Cell Biology, Chinese Academy of Sciences.
In this study, our results demonstrated that DATS, a kind of garlic-derived compounds, decreased the T24 human bladder cancer cell number in a dose- and time-dependent manner which is related to induced G2/M phase cell cycle arrest and apoptosis.