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References in periodicals archive ?
In addition to compounds related to the glycolytic pathway (dihydroxyacetone phosphate [DHAP], F-1,6-dP, lactic acid) and purine metabolism (AMP, ATP, GMP, hypoxanthine, IP, etc.), other compounds, including 6-phosphogluconic acid (6PG), sedoheptulose 7-phosphate (S7P), citric acid, fumaric acid, and NAD+, showed changes in their contents in the postmortem period.
Pure standards of guanine ([[lambda].sub.max] = 249) and hypoxanthine ([[lambda].sub.max] = 247) were analyzed in parallel.
The method developed was applied to determine urinary creatinine, uric acid, and hypoxanthine levels in children with autism.
INS-1 medium supplemented with 1 x HT (100 [micro]M hypoxanthine, 16 [micro]M thymidine) (H0137, Sigma) was used as the coculture medium.
As hypoxanthine would be transformed into uric acid with the action of XO, therefore, detection of uric acid level has been proposed to indirectly indicate XO activity [27, 38].
Verma, "A transmissible retrovirus expressing human hypoxanthine phosphoribosyltransferase (HPRT): gene transfer into cells obtained from humans deficient in HPRT," Proceedings of the National Academy of Sciences of the United States of America, vol.
Adenosine TriPhosphate (ATP) and its degradation products up to Hypoxanthine (Hx) were assessed by High-Performance Liquid Chromatography (HPLC) according to Ryder [4].
This procedure was repeated twice and the aminopterin concentration thus continuously reduced over six days, while hypoxanthine and thymidine were supplemented following manufacturer's recommendations (1: 100).
Each sample was added to a substrate mixture containing EDTA (0.05 mM), hypoxanthine (0.2 mM), and nitroblue tetrazolium (NBT, 1 mM) in 50 mM phosphate buffer (pH 7.5).
Additionally, it was found that, compared to control group, adenosine and cytidine monophosphate were elevated but their products of degradation (uracil, inosine, hypoxanthine, and xanthine) were decreased in model group, suggesting a disordered nucleotide metabolism of SD.
Purines are metabolized to hypoxanthine and xanthine via the action of enzymatic activity of xanthine oxidase to UA [24, 25].