Briefly, aliquots of 0.1 ml samples were directly applied onto modified charcoal-cefoperazone-deoxycholate agar (mCCDA) plates, supplemented with cefoperazone (32 mg/l) and amphotericin B (10 mg/l), followed by incubation at 42[degrees]C for 48 h under microaerobic conditions.
Suspected colonies on mCCDA were confirmed by duplex PCR.
FDS-NPs were validated against standard mCCDA plating method (ISO 10272:2006) with multiplex PCR confirmation following ISO 16140:2003.
The values were calculated by comparing with results from the reference method, mCCDA plating with duplex PCR confirmation (Figure 5).
The results of mCCDA plate count with PCR assay gave positive for cells less than [10.sup.3] cfu/g, but FDS-NPs displayed negative results.
Single-laboratory method validation of FDS-NPs against standard mCCDA plating with duplex PCR confirmation, following ISO16140:2003 guidelines, indicated that the FDS-MPs had a limit of detection level (LOD) of 103 cfu/ml.
jejuni colonies from the mCCDA plates of viscera contents; and lane N: sterile water was used as a negative control.
MCCDA''s Luncheon Club Supervisor Yvonne Chin said: "The allotment project has really helped us connect with the elderly Chinese residents in Liverpool who normally keep themselves to themselves and they have all really enjoyed the experience of gardening together.
Chief executive at LMH Steve Coffey said: "The work we do in the community goes much further than simply bricks and mortar and it is really rewarding to be able to support and fund organisations like the MCCDA who, in turn, are making a huge difference in society by engaging with hard to reach groups.
MCCDA won LMH''s Unity title at the Focus Awards in April.